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Hep1-6 cells

Web27 apr. 2024 · Cell culture and treatment. HepG2 and Hep1-6 cells were cultured in 6-well plates in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) in a 5% humidified CO 2 incubator at 37 °C. Web26 aug. 2024 · The murine HCC cells Hep1-6 were implanted in the liver of mouse. With oral feeding of Rg3 (10 mg/kg once a day for 30 days), the quantitative analysis of apoptosis was performed by using pathology and a transmission electron microscope and microvessel density was quantitatively measured by immunohistochemical staining of the CD105 …

Establishment of liver tumor cell lines from atherogenic and

WebOur Hepa1-6 cells are available with a variety of different reporters, including firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared … Web2 apr. 2024 · The cells were then rinsed with serum-free MEM and introduced to 1 mL of MEM which is containing 15 µg/mL Lipofectamine 2000 (Invitrogen) and 5 μg of the plasmid. After 6 h incubation 37°C, the cells were introduced to 1 mL of MEM containing 20% FCS, and then reincubated at 37°C for 18 h. corowa maternal child health https://shoptauri.com

HEPA 1-6 - German Collection of Microorganisms and Cell Cultures GmbH ...

Web22 mrt. 2024 · Rapid identification of cancer cells is crucial for ... By using LTRS, we obtained high-quality single-cell Raman spectra of normal hepatocytes (HL-7702) and liver cancer cell lines (SMMC-7721, Hep3B, HepG2, SK-Hep1 ... (877 777 6435) in the United States, or +1 212 448 2500 outside of the United States, 8:30AM to 6:00PM U.S. Eastern ... Web2.1 Cell lines and chemicals HepG2, HEK293T and Hep1-6 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Biological Industries, Kirbuta Beit Haemek, Israel), 100 U/mL of penicillin, and 100 mg/mL of WebInterestingly, tumors from D-hep cells, not Hepa1-6 cells, regressed in wild-type C57BL/6mice whereas D-hep cells exhibited similar tumorigenesis as Hep1–6 cells in immunodeficient mice. As expected, additional Hepa1-6 cells failed to form tumors in the D-hep-C57 mice in which D-hep cells were eliminated. corowa massage

FGF-21 as a potential therapeutic target of NAFLD TCRM

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Hep1-6 cells

HEPA 1-6 - German Collection of Microorganisms and Cell Cultures GmbH ...

Web7 feb. 2024 · Therefore, SK-Hep1 or Hep3B was cultured with or without NK-92 and NK-92 single culture was also included for comparison. After 6 h, NK-92 was harvested from the supernatant including non-adherent cells, and SK-Hep1 or Hep3B were harvested as cell lysates from individual culture. The expressions of CXCR3A and CXCR3B were …

Hep1-6 cells

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Web23 feb. 2016 · When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D … Web3 sep. 2024 · Hep1-6 mouse hepatoma cells were used to establish a mouse HC model in previous study of our lab ( 21 ). In addition, the histological features of HC models built by Hep1-6 cells in experimental …

Web(c) Hepa1-6 cells were treated with 10 μM Sorafenib, DMSO alone or left untreated (w/o) and analyzed at indicated time points after treatment for gene expression of Cyclins A2, … http://www.biofeng.com/ATCC/xibao/Hepa-1-6.html

Web17 sep. 2024 · The cell line human GPC3 (hGPC3)-Hepa1-6 was established by hGPC3-expressing lentivirus to infect Hepa1-6 cells. Flow cytometry analysis revealed that compared with the control cells, the expression of GPC3 in hGPC3-Hep1-6 cells was significantly upregulated (Figure 2A,B). Next, hGPC3-Hep1-6 cells were used to … Web25 feb. 2024 · Comparison of anticancer drugs in liver cancer cell lines. Male-derived SK-Hep1 and Hep3B cells, and female-derived SNU-387 and SNU-878 cells were treated with Dox, 5-FU and CDDP for 24 h. Data are presented as mean ± standard deviation (n = 6). * p < 0.05 (one-way ANOVA test followed by Tukey’s post-hoc test)

Web5 feb. 2024 · Hep1‐6 cells in the logarithmic phase of growth were digested with trypsin into cell suspensions, and cell concentrations were adjusted to 1 × 10 7 cells/mL. Four‐week‐old C57/BL mice ( n = 12) were provided by the Animal Supply Center of Zhejiang Academy of Medical Science [Hangzhou, China; certificate no. SCXK (Zhe) …

WebHepa 1-6 [Hepa1-6] CRL-1830 ™ Product category Animal cells Organism Mus musculus, mouse Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod Morphology epithelial Tissue Liver Disease Hepatoma Applications 3D cell culture … See all new products See what's coming next. ATCC (American Type Culture … ATCC is working to harmonize our Safety Data Sheet (SDS) distribution with … corowa maternal and child health nurseWeb13 apr. 2024 · The overexpression of SREBP1 and knockdown of DDX39B in HepG2, SK-Hep1 and MHCC97H cells were confirmed by qPCR and western blot assays (Fig. 6A-B and Supplementary Fig. S5A-S5B). SREBP1 overexpression effectively restored the decreased expression of lipogenic enzymes (ACLY, FASN, ACACA and SCD1) caused … faotoushopWeb15 dec. 2016 · The HEP1-6 cells were co-transfected with the pmiRGLO vector, pmiRGLO-PP4R1 and a miR-338-3p mimic (or negative control) using Effectene (Qiagen, Hilden, North Rhine-Westphalia, Germany) for 48 h. Luciferase reporter assays were performed by using the Dual-Glo Luciferase assay system (Promega, Madison, WI). Chromatin … faoug hotelsWebThe cell line is aneuploid human (XX), with chromosome counts in the hypotriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N7, N12, and N17 tend to be over-represented in some metaphases. fao tree spacingWeb细胞凋亡检测试剂盒 细胞房除菌系列 50mL瓶装特级胎牛血清 规格：50mL/50mLx10 价格：￥450/￥3400 选购理由 50mL独立包装，告别分液，降低污染风险和研究结果受损机率 … faoug plageWebOriginally described as originating from an adenocarcinoma of liver and thus classified as hepatocellular carcinoma. Later studies show that it most probably has arisen from endothelial cells (PubMed=1371504; PubMed=31938418). Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). corowa meatsWeb5 jun. 2024 · 将质粒送与南京金斯瑞公司进行进 侵染Hep1-6细胞,经过blasticidin筛选30 d,收集部 一步测序鉴定,结果显示目的基因序列正确。 分细胞进行qPCR和Western blotting检测。 与对照 组比较,Nrf2 mRNA水平升高了2倍 (图3A),蛋白 水平也有明显升高 (图3B、3C)。 表明Nrf2 过表达 Hep1-6稳转株构建成功。 2.4 Nrf2 敲减稳转株的筛选与鉴定 制备装载有 Nrf2 … corowa medical clinic doctors